Veracious, who was due to be the mount of Frankie Dettori, has been ruled out of the Qipco 1000 Guineas due to a "training setback".
The Sir Michael Stoute-trained daughter of Frankel was an impressive winner of a Newmarket maiden last October and had impressed Dettori in a gallop before racing at last week's Craven meeting.
In a statement to Press Association Sport, Stoute said: "Veracious will not be running in the 1000 Guineas because she has had a training setback."
Veracious had been as low as 6-1 for the Newmarket Classic on May 6.
Chris Richardson, managing director of owner Cheveley Park Stud, confirmed Veracious is lame and expects her to undergo further tests in the coming days
He said: "I spoke to Sir Michael Stoute and I don't know exactly what it is, but she is not 100 per cent right.
"She is lame in behind and I think they are going to do some more tests and examinations to try to establish what it is.
"Frankie Dettori was very happy with her gallop on Wednesday and it looked good. We are all very disappointed."